polyclonal mouse anti-human cd3  (Agilent technologies)

Journal: PLoS ONE

Article Title: Immunopathogenesis of canine chronic ulcerative stomatitis

doi: 10.1371/journal.pone.0227386

Figure Lengend Snippet: Primary antibodies used in this study.

Article Snippet: Samples were further blocked with normal donkey serum and FcR Blocking Reagent (Miltenyi) and incubated overnight with rabbit anti-human CD3 polyclonal antibody (1:50 dilutio) and goat antihuman IL17 polyclonal antibody (1:100 dilution) or rat anti-FoxP3 (1:200 dilution).

Techniques: Biomarker Discovery, Transgenic Assay

Journal: PLoS ONE

Article Title: Immunopathogenesis of canine chronic ulcerative stomatitis

doi: 10.1371/journal.pone.0227386

Figure Lengend Snippet: IHC Semi-quantitative scoring system.

Article Snippet: Samples were further blocked with normal donkey serum and FcR Blocking Reagent (Miltenyi) and incubated overnight with rabbit anti-human CD3 polyclonal antibody (1:50 dilutio) and goat antihuman IL17 polyclonal antibody (1:100 dilution) or rat anti-FoxP3 (1:200 dilution).

Techniques: Immunohistochemistry, Clinical Proteomics

Journal: PLoS ONE

Article Title: Immunopathogenesis of canine chronic ulcerative stomatitis

doi: 10.1371/journal.pone.0227386

Figure Lengend Snippet: A -Normal canine oral mucosa illustrates very few CD3+ T cells (white arrows) that are distributed at the mucosal–submucosal interface (A, stitched 20x tile image); B -Co-labeling of CD3 (green) and FoxP3 (red) reveals abundant FoxP3+ T cells that are noted with the submucosa of CCUS lesions; C-IL17 (red) and CD3(green) co-staining reveals marked infiltration of sub-epithelial and mucosal tissues with CD3+T cells and IL17 producing cells. Note that most of the IL17 producing cells (i.e. red) are not double labeled with CD3 (i.e. green) indicating that IL17 producing cells are primarily non-T cells. D—Close up (630x) image illustrates a single positive, IL17, cell (white arrow), a single positive CD3 T cell (white arrow head) and a group of double positive (i.e. IL17/CD3) IL17 producing T cells (asterisk). E—No significant differences were identified in the frequencies of CD3+/FoxP3+ cells, CD3+ cells, IL17+ cells and DP positive cells between the three histologic variants.

Article Snippet: Samples were further blocked with normal donkey serum and FcR Blocking Reagent (Miltenyi) and incubated overnight with rabbit anti-human CD3 polyclonal antibody (1:50 dilutio) and goat antihuman IL17 polyclonal antibody (1:100 dilution) or rat anti-FoxP3 (1:200 dilution).

Techniques: Labeling, Staining

Journal: PLoS ONE

Article Title: Immunopathogenesis of canine chronic ulcerative stomatitis

doi: 10.1371/journal.pone.0227386

Figure Lengend Snippet: A-There was no statistically significant difference in the number of infiltrating T cells; B-IL17+ cells; C and D-percent double positive (IL17+/CD3+); and E-Treg cells. F-A negative correlation was found between the numbers of IL17+ cells per 20x field and the stomatitis disease activity index.

Article Snippet: Samples were further blocked with normal donkey serum and FcR Blocking Reagent (Miltenyi) and incubated overnight with rabbit anti-human CD3 polyclonal antibody (1:50 dilutio) and goat antihuman IL17 polyclonal antibody (1:100 dilution) or rat anti-FoxP3 (1:200 dilution).

Techniques: Activity Assay